Methods & instrumentation.

HPLC-UV Tandem ESI-QTOF-MS
Analytical testing & contract research
Mission Hills, California

Every number this laboratory reports comes from a named instrument and method, and is judged by rules it publishes. This page states them: the bench, the decision rules, and the practice around reference standards. A method that can be checked is worth more than a mark that has to be trusted.

A liquid chromatography stack connected by capillary tubing to a benchtop mass spectrometer on a laboratory bench — Liquid chromatograph and mass spectrometer, as the two sit together on a bench · generated illustration — not a photograph of this laboratory

Instrument 01

HPLC-UV Tandem ESI-QTOF-MS

Liquid chromatography separates the sample, a UV detector measures what elutes, and quadrupole time-of-flight mass spectrometry measures its accurate mass. Purity is read from the UV trace and identity from the mass.

Instrument 02

Fluorometer

A fluorometric LAL (Limulus amebocyte lysate) assay quantifies bacterial endotoxin in the sample. LAL detects endotoxin from Gram-negative bacteria. It does not detect every pyrogen. Endotoxin testing is temporarily not available.

Instrument 03

ICP-MS

Inductively coupled plasma mass spectrometry measures heavy metals and other elemental impurities at trace levels, element by element.

How a result is judged.

The number and the method
the verdict printed beside them
never in place of them

Purity is area-normalized. Identity is accurate mass against a reference standard. Quantity is recovered mass against the label claim. In every case the certificate carries the number and the method that produced it. Its verdict, such as PASS, is printed beside the measured values and the method, never in place of them.

A schematic line drawing of a chromatogram: one tall peak with four smaller peaks along a flat baseline — Schematic chromatogram · one main peak and the smaller ones reported beside it · drawn figure, no data — not a result from this laboratory

A schematic line drawing of a mass spectrum: a tall stick with an isotope cluster beside it and scattered shorter sticks along the baseline — Schematic mass spectrum · a dominant ion and its isotope cluster · drawn figure, no data — not a result from this laboratory

No badge stands in for a number

Nothing on these pages is a certification mark, and no badge is asked to stand in for a number. The case for believing a result is the method that produced it — instrument, wavelength, reference standard, and error, printed on the certificate where they can be checked. A laboratory that publishes its decision rules can be argued with. That is the point.

Contract research.

Method development · validation
regulatory data packages
small molecule · protein · peptide

The same bench takes commissioned work, backed by more than 15 years of research experience. Each project is scoped with you, and you are kept up to date at each step.

Method development & validation

Assay development, optimization, and validation built specifically for your project — documented as it is built, so the method leaves here usable by someone who is not us.

Development · validation · troubleshooting

Small-molecule profiling

Molecular mass determination, structural elucidation and fragmentation analysis, purity and impurity profiling, and degradation studies on synthetic and natural compounds.

Accurate mass · MS/MS fragmentation · impurities

Protein & peptide profiling

Intact mass analysis, LC-MS/MS peptide mapping, identification of post-translational modifications, and sequence confirmation — the proteomics discipline behind the peptide bench.

Intact mass · peptide mapping · PTM identification

Regulatory data packages

The method as written — instrument parameters, sample preparation, and the decision rule the result was judged by.
The validation record for that method, on the matrix it was validated for — not a general claim of soundness.
Traceability from injection to reported number, so a reviewer can reproduce the work rather than take the conclusion on faith.
Prepared to support your submission. What we hand over is the data package — the regulatory argument remains yours to make.

Commission an analysis

A stainless rack of small sealed glass vials holding white powder, standing beside a cold cabinet — Reference standards in cold storage · identity is read against one, and the certificate names it · generated illustration — not a photograph of this laboratory

Reference standards

Identity is read against a reference standard, and the certificate names the standard it was read against.
Where no standard is on file, the certificate says so and states the basis of identification instead — it does not imply a comparison that did not happen.
For commissioned work, the method plan states which standard will be used and where it comes from — settled before the run, not after.

15336 Devonshire Street, Unit 6
Mission Hills, CA 91345
(747) 232-0267
main@premieranalytical.com
Mon–Fri · 10:00 AM – 6:00 PM PT

One client, quoted exactly.

Prof. Gagik Melikyan
California State University, Northridge
three phrases · nothing added

Phrase 01 · the data

“High-res MS spectra”

Said of the mass-spectrometry work, the QTOF bench this page begins with.

G. Melikyan · CSU Northridge

Phrase 02 · the pace

“Short turnaround”

Said of how quickly commissioned work came back.

G. Melikyan · CSU Northridge

Phrase 03 · the people

“Courteous, professional personnel”

Said of the people who did the work and answered for it.

G. Melikyan · CSU Northridge